Mavrilas Dimosthenis, Koutsoukos Petros G, Koletsis Efstratios N, Apostolakis Efstratios, Dougenis Dimitrios
Laboratory of Biomechanics and Biomedical Engineering, Department of Mechanical Engineering and Aeronautics, University of Patras, Patras, Greece.
Exp Biol Med (Maywood). 2006 Dec;231(11):1712-7. doi: 10.1177/153537020623101107.
Bovine pericardium (BPC) and polytetrafluoroethylene (PTFE) have been widely used to reinforce staple lines in lung resection. Since limited information regarding the calcification of these biomaterials is available, we undertook an in vitro study to evaluate their calcification potential. Commercially available BPC and PTFE biomaterials were evaluated and compared with custom-prepared BPC tissue. In vitro calcification was performed via submersion in supersaturated solution in a double-walled glass reactor at 37.0 degrees C +/- 0.1 degrees C, pH 7.4 +/- 0.1, mimicking most ion concentrations of human blood plasma. In processing of calcification, the pH decrease of the solution simulated the addition of consumed H(+), Ca(2+), and PO(4)(3-) ions from titrant solutions, the concentrations of which were based on the stoichiometry of octacalcium phosphate. The molar ion addition with time was recorded, and the initial slope of the curve was computed for each experiment. The rate of calcification developed (molar calcium phosphate ion addition rate per time and total surface area) (R) was computed after that with respect to the relative supersaturation (sigma) used in each experiment. R for custom-prepared BPC tissues was found to be in the range of 0.19 +/- 0.08 to 0.52 +/- 0.19 (n = 17) in sigma range of 0.72 to 1.42. Commercial BPC was found to be 0.016 to 0.052 (n = 4), and PTFE was 0.005 to 0.05 (n = 8) in the same sigma range. Both clinically applied biomaterials, BPC and PTFE, seemed to be calcified with rates of at least one order of magnitude lower than the custom-prepared BPC tissue. This data suggested that BPC and PTFE biomaterials showed a similar, relatively very low tendency for calcification compared with custom-prepared BPC tissue. Although further studies are necessary, staple line reinforcement by these two biomaterials should be considered safe from the calcification point of view.
牛心包（BPC）和聚四氟乙烯（PTFE）已被广泛用于加强肺切除术中的吻合钉缝线。由于关于这些生物材料钙化的信息有限，我们进行了一项体外研究以评估它们的钙化潜力。对市售的BPC和PTFE生物材料进行了评估，并与定制制备的BPC组织进行了比较。体外钙化是通过将其浸没在双壁玻璃反应器中的过饱和溶液中进行的，温度为37.0℃±0.1℃，pH值为7.4±0.1，模拟人血浆的大多数离子浓度。在钙化过程中，溶液的pH值下降模拟了滴定剂溶液中消耗的H⁺、Ca²⁺和PO₄³⁻离子的添加，其浓度基于磷酸八钙的化学计量。记录随时间的摩尔离子添加量，并计算每个实验曲线的初始斜率。之后根据每个实验中使用的相对过饱和度（σ）计算钙化发展速率（每时间和总表面积的摩尔磷酸钙离子添加速率）（R）。在σ范围为0.72至1.42时，发现定制制备的BPC组织的R在0.19±0.08至0.52±0.19范围内（n = 17）。在相同的σ范围内，市售BPC为0.016至0.052（n = 4），PTFE为0.005至0.05（n = 8）。临床应用的两种生物材料BPC和PTFE似乎都有钙化现象，其速率比定制制备的BPC组织至少低一个数量级。该数据表明，与定制制备的BPC组织相比，BPC和PTFE生物材料显示出相似的、相对非常低的钙化倾向。尽管需要进一步研究，但从钙化角度考虑，用这两种生物材料加强吻合钉缝线应该是安全的。
