采用分批法和流动注射法分光光度法测定氟西汀。

Spectrophotometric determination of fluoxetine by batch and flow injection methods.

作者信息

Afkhami Abbas, Madrakian Tayyebeh, Khalafi Lida

机构信息

Faculty of Chemistry, Bu-Ali-Sina University, Hamadan 65174, Iran.

出版信息

Chem Pharm Bull (Tokyo). 2006 Dec;54(12):1642-6. doi: 10.1248/cpb.54.1642.

DOI:10.1248/cpb.54.1642

PMID:17139097

Abstract

A rapid, simple, and accurate spectrophotometric method is presented for the determination of fluoxetine by batch and flow injection analysis methods. The method is based on fluoxetine competitive complexation reaction with phenolphthalein-beta-cyclodextrin (PHP-beta-CD) inclusion complex. The increase in the absorbance of the solution at 554 nm by the addition of fluoxetine was measured. The formation constant for fluoxetin-beta-CD was calculated by non-linear least squares fitting. Fluoxetine can be determined in the range 7.0 x 10(-6)-2.4 x 10(-4) mol l(-1) and 5.0 x 10(-5)-1.0 x 10(-2) mol l(-1) by batch and flow methods, respectively. The limit of detection and limit of quantification were respectively 4.13 x 10(-6) mol l(-1) and 1.38 x 10(-5) mol l(-1) for batch and 2.46 x 10(-5) mol l(-1) and 8.22 x 10(-5) mol l(-1) for flow method. The sampling rate in flow injection analysis method was 80+/-5 samples h(-1). The method was applied to the determination of fluoxetine in pharmaceutical formulations and after addition to human urine samples.

摘要

本文介绍了一种通过分批和流动注射分析法测定氟西汀的快速、简单且准确的分光光度法。该方法基于氟西汀与酚酞-β-环糊精（PHP-β-CD）包合物的竞争络合反应。通过测量加入氟西汀后溶液在554nm处吸光度的增加来进行测定。通过非线性最小二乘法拟合计算氟西汀-β-环糊精的形成常数。分批法和流动法测定氟西汀的线性范围分别为7.0×10⁻⁶ - 2.4×10⁻⁴ mol/L和5.0×10⁻⁵ - 1.0×10⁻² mol/L。分批法的检测限和定量限分别为4.13×10⁻⁶ mol/L和1.38×10⁻⁵ mol/L，流动法的检测限和定量限分别为2.46×10⁻⁵ mol/L和8.22×10⁻⁵ mol/L。流动注射分析法的采样率为80±5个样品/小时。该方法应用于药物制剂中氟西汀的测定以及加样后的人尿样分析。