Wendelboe Aaron Mark, Van Rie Annelies
AMW, 1190 St. Francis Dr., Suite N1320, Santa Fe, NM 87505, USA.
Expert Rev Mol Diagn. 2006 Nov;6(6):857-64. doi: 10.1586/14737159.6.6.857.
The accurate and timely diagnosis of pertussis continues to be challenging. The widespread use of pertussis vaccines has dramatically altered the epidemiology and clinical presentation of pertussis disease, such that many cases do not present with the hallmark symptoms, such as inspiratory whoop, post-tussive vomiting and paroxysmal cough. A variety of laboratory tools are available to aid in the diagnosis of pertussis, including culture, direct fluorescent antibody testing, PCR, and paired and single serology techniques. This article reviews the strengths and limitations, including the sensitivity and specificity, of each of these diagnostic tools.
百日咳的准确及时诊断仍然具有挑战性。百日咳疫苗的广泛使用极大地改变了百日咳疾病的流行病学和临床表现，以至于许多病例并不表现出标志性症状，如吸气性哮吼、咳嗽后呕吐和阵发性咳嗽。有多种实验室检测方法可辅助百日咳的诊断，包括培养、直接荧光抗体检测、聚合酶链反应（PCR）以及双份和单份血清学技术。本文综述了这些诊断方法各自的优势和局限性，包括敏感性和特异性。
