G蛋白介导的信号转导的发现：个人记述以及对促成我们当前理解的最初发现与贡献的概述。
The discovery of signal transduction by G proteins: a personal account and an overview of the initial findings and contributions that led to our present understanding.
作者信息
Birnbaumer Lutz
机构信息
Division of Intramural Research, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA.
出版信息
Biochim Biophys Acta. 2007 Apr;1768(4):756-71. doi: 10.1016/j.bbamem.2006.09.027. Epub 2006 Oct 4.
Abstract
The realization that there existed a G-protein coupled signal transduction mechanism developed gradually and was initially the result of an ill fated quest for uncovering the mechanism of action of insulin, followed by a refocused research in many laboratories, including mine, on how GTP acted to increase hormonal stimulation of adenylyl cyclase. Independent research into how light-activated rhodopsin triggers a response in photoreceptor cells of the retina and the attendant biochemical studies joined midway and, without the left hand knowing well what the right hand was doing, preceded classical G protein research in identifying the molecular players responsible for signal transduction by G proteins.
摘要
人们逐渐认识到存在一种G蛋白偶联信号转导机制，最初这是对胰岛素作用机制进行探索时遭遇挫折的结果，随后包括我所在实验室在内的许多实验室将研究重点重新聚焦于GTP如何作用以增强激素对腺苷酸环化酶的刺激。关于光激活视紫红质如何在视网膜光感受器细胞中引发反应的独立研究以及相关生化研究中途加入进来，而且在左手不太清楚右手在做什么的情况下，在确定负责G蛋白信号转导的分子参与者方面先于经典G蛋白研究。
相似文献
1
The discovery of signal transduction by G proteins: a personal account and an overview of the initial findings and contributions that led to our present understanding.
Biochim Biophys Acta. 2007 Apr;1768(4):756-71. doi: 10.1016/j.bbamem.2006.09.027. Epub 2006 Oct 4.
2
Pertussis toxin-catalyzed ADP-ribosylation of adenylate cyclase. Effects of guanyl nucleotides and rhodopsin.
Dev Biol Stand. 1985;61:43-9.
3
Regulation of guanine nucleotide turnover on Gi/Go by agonist-stimulated and spontaneously active muscarinic receptors in cardiac membranes.
Arch Biochem Biophys. 1999 Jan 1;361(1):57-64. doi: 10.1006/abbi.1998.0945.
4
Estimation of agonist activity at G protein-coupled receptors: analysis of M2 muscarinic receptor signaling through Gi/o,Gs, and G15.
J Pharmacol Exp Ther. 2007 Jun;321(3):1193-207. doi: 10.1124/jpet.107.120857. Epub 2007 Mar 28.
5
Alfred Goodman Gilman (1941-2015).
Nature. 2016 Jan 21;529(7586):284. doi: 10.1038/529284a.
6
Redox modulation of Gi protein expression and adenylyl cyclase signaling: role of nitric oxide.
Antioxid Redox Signal. 2004 Apr;6(2):385-92. doi: 10.1089/152308604322899459.
7
Functional analyses of type V adenylyl cyclase.
Methods Enzymol. 2002;345:160-87. doi: 10.1016/s0076-6879(02)45015-x.
8
Gonadotropin-releasing hormone signaling pathways in an experimental ovarian tumor.
Endocrinology. 2003 Jul;144(7):2957-66. doi: 10.1210/en.2003-0011.
9
Expansion of signal transduction by G proteins. The second 15 years or so: from 3 to 16 alpha subunits plus betagamma dimers.
Biochim Biophys Acta. 2007 Apr;1768(4):772-93. doi: 10.1016/j.bbamem.2006.12.002. Epub 2006 Dec 15.
10
Studies on nucleotide and receptor regulation of Gi proteins: effects of pertussis toxin.
Mol Endocrinol. 1989 Jul;3(7):1115-24. doi: 10.1210/mend-3-7-1115.
引用本文的文献
1
From theory to platelets: unraveling the history and complexities of biased signaling.
Blood Vessel Thromb Hemost. 2025 May 5;2(3):100073. doi: 10.1016/j.bvth.2025.100073. eCollection 2025 Aug.
2
Milestones in the development and implementation of FRET-based sensors of intracellular signals: A biological perspective of the history of FRET.
Cell Signal. 2020 Nov;75:109769. doi: 10.1016/j.cellsig.2020.109769. Epub 2020 Sep 6.
3
Modulatory effect of laser irradiation on mastoparan-7-induced contraction.
Biomed Rep. 2020 Jan;12(1):23-29. doi: 10.3892/br.2019.1255. Epub 2019 Nov 11.
4
Optical approaches for single-cell and subcellular analysis of GPCR-G protein signaling.
Anal Bioanal Chem. 2019 Jul;411(19):4481-4508. doi: 10.1007/s00216-019-01774-6. Epub 2019 Mar 30.
5
In Silico Studies Targeting G-protein Coupled Receptors for Drug Research Against Parkinson's Disease.
Curr Neuropharmacol. 2018;16(6):786-848. doi: 10.2174/1570159X16666180308161642.
6
When Heterotrimeric G Proteins Are Not Activated by G Protein-Coupled Receptors: Structural Insights and Evolutionary Conservation.
Biochemistry. 2018 Jan 23;57(3):255-257. doi: 10.1021/acs.biochem.7b00845. Epub 2017 Oct 16.
7
Developmental regulators in Aspergillus fumigatus.
J Microbiol. 2016 Mar;54(3):223-31. doi: 10.1007/s12275-016-5619-5. Epub 2016 Feb 27.
8
Registered report: Diverse somatic mutation patterns and pathway alterations in human cancers.
Elife. 2016 Feb 19;5:e11566. doi: 10.7554/eLife.11566.
9
Biased agonism: An emerging paradigm in GPCR drug discovery.
Bioorg Med Chem Lett. 2016 Jan 15;26(2):241-250. doi: 10.1016/j.bmcl.2015.12.024. Epub 2015 Dec 9.
10
From GTP and G proteins to TRPC channels: a personal account.
J Mol Med (Berl). 2015 Sep;93(9):941-53. doi: 10.1007/s00109-015-1328-5. Epub 2015 Sep 16.
本文引用的文献
1
Light-induced, reversible binding of proteins to bovine photoreceptor membranes. Influence of nucleotides.
Neurochem Int. 1980;1C:269-85. doi: 10.1016/0197-0186(80)90066-2.
2
Mammalian G proteins and their cell type specific functions.
Physiol Rev. 2005 Oct;85(4):1159-204. doi: 10.1152/physrev.00003.2005.
3
4
Characterization of bovine rod outer segment G-protein.
J Biol Chem. 1982 Jun 10;257(11):6452-60.
5
Islet-activating protein. A modifier of receptor-mediated regulation of rat islet adenylate cyclase.
J Biol Chem. 1981 Aug 25;256(16):8310-7.
6
Direct modification of the membrane adenylate cyclase system by islet-activating protein due to ADP-ribosylation of a membrane protein.
Proc Natl Acad Sci U S A. 1982 May;79(10):3129-33. doi: 10.1073/pnas.79.10.3129.
7
Purification of the regulatory component of adenylate cyclase.
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6516-20. doi: 10.1073/pnas.77.11.6516.
8
9
Identification of a gamma subunit associated with the adenylyl cyclase regulatory proteins Ns and Ni.
J Biol Chem. 1984 Feb 25;259(4):2039-42.
10
The subunits of the stimulatory regulatory component of adenylate cyclase. Resolution of the activated 45,000-dalton (alpha) subunit.
J Biol Chem. 1983 Sep 25;258(18):11369-76.
Zotero 插件