Grześk Elżbieta, Mackiewicz-Milewska Magdalena, Mackiewicz-Nartowicz Hanna, Wiciński Michał, Burdziński Igor, Korsak Maryia, Kopczyńska Anna, Hagner Wojciech, Grześk Grzegorz
Department of Pediatrics, Hematology and Oncology, Faculty of Medicine, Collegium Medicum, Nicolaus Copernicus University, 85-094 Bydgoszcz, Poland.
Department of Rehabilitation, Faculty of Health Sciences, Collegium Medicum, Nicolaus Copernicus University, 85-094 Bydgoszcz, Poland.
Biomed Rep. 2020 Jan;12(1):23-29. doi: 10.3892/br.2019.1255. Epub 2019 Nov 11.
Mastoparan-7 activates guanine nucleotide-binding proteins (G-proteins) and stimulates both apoptosis and increases in cytoplasmic calcium concentration and may induce smooth muscle contraction. The primary aim of the present study was to evaluate the modulatory effect of laser stimulation on vascular smooth muscle contraction induced by direct stimulation of G-protein with mastoparan-7. Experiments were performed on isolated and perfused tail arteries of Wistar rats. Contraction force in the model was measured by increased levels of perfusion pressure with a constant flow. Irradiation treatment was applied directly to the blood vessels. The laser was applied in increasing doses of 10 mW (E=1.8 J), 30 mW (E=5.5 J) and 110 mW (E=19.8 J). Time of exposure was 3 min for each irradiation. In the laser-stimulated arteries, a significant and dose-dependent decrease was observed. The half maximal effective concentration values were 4.43±2.2x10, 2.4±0.56x10, 3.2±0.72x10 and 7.7±0.3x10 M/l in the control and 10, 30 and 110 mW laser irradiation groups, respectively. Significant (P<0.001) changes were identified for all laser treatment groups in comparison with the control. When analyzing the function of calcium ion (Ca) stores was analyzed, a significant inhibition of influx from both intra- and extracellular Ca stores was observed. The results from the present study suggested that contraction induced by direct activation of G-protein with mastoparan-7 may by effectively inhibited by laser radiation, and that the effect was associated with an inhibition of Ca influx from both intracellular and extracellular Ca stores.
马斯托帕兰 -7 激活鸟嘌呤核苷酸结合蛋白（G 蛋白），刺激细胞凋亡并增加细胞质钙浓度，还可能诱导平滑肌收缩。本研究的主要目的是评估激光刺激对用马斯托帕兰 -7 直接刺激 G 蛋白诱导的血管平滑肌收缩的调节作用。实验在 Wistar 大鼠分离并灌注的尾动脉上进行。通过在恒定流量下灌注压力水平的增加来测量模型中的收缩力。直接对血管进行照射处理。激光以 10 mW（能量 = 1.8 J）、30 mW（能量 = 5.5 J）和 110 mW（能量 = 19.8 J）的递增剂量施加。每次照射的暴露时间为 3 分钟。在激光刺激的动脉中，观察到显著且剂量依赖性的降低。对照组以及 10、30 和 110 mW 激光照射组的半数最大有效浓度值分别为 4.43±2.2x10、2.4±0.56x10、3.2±0.72x10 和 7.7±0.3x10 M/l。与对照组相比，所有激光治疗组均有显著（P<0.001）变化。在分析钙离子（Ca）储存功能时，观察到细胞内和细胞外 Ca 储存的流入均受到显著抑制。本研究结果表明，用马斯托帕兰 -7 直接激活 G 蛋白诱导的收缩可被激光辐射有效抑制，且该效应与抑制细胞内和细胞外 Ca 储存的 Ca 流入有关。
