TRIP-Br家族的转录调控与亚细胞调控

Transcriptional and subcellular regulation of the TRIP-Br family.

作者信息

Lai I-Lu, Wang Shi-Yun, Yao Ya-Li, Yang Wen-Ming

机构信息

Institute of Molecular Biology, National Chung Hsing University, 250 Kuo-Kuang Road, Taichung 40227, Taiwan.

出版信息

Gene. 2007 Feb 15;388(1-2):102-9. doi: 10.1016/j.gene.2006.10.008. Epub 2006 Oct 24.

DOI:10.1016/j.gene.2006.10.008

PMID:17141982

Abstract

TRIP-Brs are a recently discovered set of proteins whose functions remain poorly characterized. Here we report the identification of TRIP-Br3 as a member of the TRIP-Br family along with evidence showing that TRIP-Brs interact with bromodomain-containing transcriptional cofactors PCAF, STAF65gamma, and KAP1. PCAF, a histone acetyltransferase; STAF65gamma, a protein associated with histone acetylation activity; and KAP1, a corepressor, influence the transcriptional activity of TRIP-Brs differentially. Finally, while all three TRIP-Brs are localized to the nucleus, TRIP-Br2 and TRIP-Br3 are also present in the cytoplasm through interaction with CRM1. Our results suggest that different TRIP-Brs function by interacting with a wide variety of bromodomain-containing transcriptional regulators in different subcellular locales.

摘要

TRIP-Brs是最近发现的一组蛋白质，其功能仍未得到充分表征。在此，我们报告了TRIP-Br3作为TRIP-Br家族成员的鉴定结果，同时有证据表明TRIP-Brs与含溴结构域的转录辅因子PCAF、STAF65γ和KAP1相互作用。PCAF是一种组蛋白乙酰转移酶；STAF65γ是一种与组蛋白乙酰化活性相关的蛋白质；KAP1是一种共抑制因子，它们对TRIP-Brs转录活性的影响各不相同。最后，虽然所有三种TRIP-Brs都定位于细胞核，但TRIP-Br2和TRIP-Br3也通过与CRM1相互作用而存在于细胞质中。我们的结果表明，不同的TRIP-Brs通过在不同亚细胞区域与多种含溴结构域的转录调节因子相互作用来发挥功能。