Robitaille G, Sullivan R, Bleau G
Department of Animal Science, McGill University, Ste-Anne de Bellevue, Montreal, Canada.
J Exp Zool. 1991 Apr;258(1):69-74. doi: 10.1002/jez.1402580108.
The electrophoretic analysis of the proteins that were extracted from immature caput and mature cauda sperm showed evidence of accumulation of several proteins during the epididymal transit of the sperm. An antiserum, raised against detergent-extracted proteins from mature spermatozoa, immunostained six epididymal proteins with apparent molecular masses of 16, 22.5, 26, 37, 60, and 80 kDa on Western blots of epididymal fluid. Of these proteins, only the 26 kDa protein was significantly immunodetected in proximal caput epididymal fluid. Its biosynthesis by caput epididymis was confirmed by immunoprecipitation of an in vitro translated product of caput poly (A) RNA. The homology of the 26 kDa epididymal protein with the 26 kDa sperm protein was verified by epitope mapping. The other epididymal proteins were found in the fluid of the more distal portions of the organ. Their presence in the epididymal fluid coincided with their detection on the sperm. These epididymal proteins were considered to be sperm-coating proteins.
对从未成熟的头部精子和成熟的尾部精子中提取的蛋白质进行的电泳分析表明，精子在附睾转运过程中有几种蛋白质积累的迹象。用从成熟精子中经去污剂提取的蛋白质制备的抗血清，在附睾液的蛋白质免疫印迹上对六种表观分子量分别为16、22.5、26、37、60和80 kDa的附睾蛋白进行了免疫染色。在这些蛋白质中，只有26 kDa的蛋白质在附睾近端头部液中被显著免疫检测到。通过对附睾头部聚腺苷酸RNA体外翻译产物的免疫沉淀，证实了附睾头部对其生物合成。通过抗原表位作图验证了26 кDa附睾蛋白与26 кDa精子蛋白的同源性。其他附睾蛋白存在于器官更远端部分的液体中。它们在附睾液中的存在与在精子上的检测结果一致。这些附睾蛋白被认为是精子包被蛋白。
