Ruíz J M, Medrano M, Alió J L
Division of Ophthalmology, Faculty of Medicine, University of Alicante, Spain.
Ophthalmic Res. 1991;23(1):27-30. doi: 10.1159/000267083.
We present an improved and simplified method for vital staining of the corneal endothelium, which allows high-quality images that are excellent for morphological and morphometric studies. This method is based upon the combination of trypan blue and alizarin red S. The main advantages of this technique are simplicity, rapidity and low cost.
我们提出了一种用于角膜内皮活体染色的改进且简化的方法,该方法能生成高质量图像,非常适合形态学和形态计量学研究。此方法基于台盼蓝和茜素红S的组合。该技术的主要优点是简单、快速且成本低。