Dong Juan'e, Liang Zongsuo, Wei Qin, Chen Shenglong
Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling.
Ying Yong Sheng Tai Xue Bao. 2006 Sep;17(9):1613-8.
By the method of HPLC, and with indigo and indirubin as indicative constituents, this paper determined the effective constituents contents in indigowoad roots and leaves collected from different regions. The results showed that the total content of effective constituents in indigowoad root was the highest (16.27 mg x kg(-1)) from Hanyin of Shaanxi Province, followed by Longde of Ningxia Autonomous Region (15.67 mg x kg(-1)), Bozhou of Anhui Province (14.90 mg x kg(-1)), and Linquan of Anhui Province and Jiamusi of Heilongjiang Province (13.97 mg x kg(-1)), and that in indigowoad leaf had the same order, with the values being 698.32, 683.68, 680.11, 654.19 and 642.23 mg x kg(-1), respectively. The HPLC chromatograms of leaf samples collected from different regions were differed, not only in the test constituents, but also in the other constituents manifested as various peaks in the chromatogram. It was suggested that besides meteorological factors, the selection of geo-authentic region should be also involved in the cultivation of Isatis indigotica.
采用高效液相色谱法，以靛蓝和靛玉红为指标成分，测定了不同产地菘蓝根和叶中有效成分的含量。结果表明，陕西省汉阴产菘蓝根中有效成分总含量最高（16.27 mg·kg⁻¹），其次是宁夏回族自治区隆德（15.67 mg·kg⁻¹）、安徽省亳州（14.90 mg·kg⁻¹）、安徽省临泉和黑龙江省佳木斯（13.97 mg·kg⁻¹）；菘蓝叶中有效成分总含量排序相同，分别为698.32、683.68、680.11、654.19和642.23 mg·kg⁻¹。不同产地叶样品的高效液相色谱图存在差异，不仅在所测成分上，其他成分在色谱图上也表现为不同的峰。提示菘蓝栽培除考虑气象因素外，还应重视道地产区的选择。
