Chen Lingyun, Subirade Muriel
Institut de recherche sur les nutraceutiques et les aliments fonctionnels (INAF/STELA), Université Laval, Sainte-Foy, Canada.
Eur J Pharm Biopharm. 2007 Mar;65(3):354-62. doi: 10.1016/j.ejpb.2006.10.012. Epub 2006 Oct 21.
The effect of preparation conditions on nutrient release from alginate (AL)-whey protein isolate (WPI) granular microspheres obtained by an emulsification/internal cold gelation method was studied by varying WPI/AL ratio, microsphere diameter, total polymer concentration and riboflavin loading. Microsphere size distribution and nutrient encapsulation efficiency (EE) were examined. Riboflavin release profiles were investigated in simulated gastric and intestinal fluids. Values for EE above 80% were obtained for most microspheres, with the notable exceptions of high AL or pure AL. Variations in WPI/AL ratio, granule size and nutrient loading have major impact on nutrient release. Microspheres prepared with a WPI/AL ratio of 8:2, a riboflavin concentration of 1% in the initial aqueous phase and diameters near 94 microm retained the vitamin in SGF and released it in SIF. By careful process design, granular microspheres with potential as oral delivery vehicles for bioactive compounds may be developed.
通过改变乳清蛋白分离物（WPI）/海藻酸盐（AL）比例、微球直径、总聚合物浓度和核黄素负载量，研究了制备条件对采用乳化/内部冷胶凝法获得的海藻酸盐（AL）-乳清蛋白分离物（WPI）颗粒微球营养物质释放的影响。检测了微球尺寸分布和营养物质包封效率（EE）。在模拟胃液和肠液中研究了核黄素释放曲线。大多数微球的EE值高于80%，高AL或纯AL微球明显除外。WPI/AL比例、颗粒大小和营养物质负载量的变化对营养物质释放有重大影响。以8:2的WPI/AL比例、初始水相中1%的核黄素浓度和接近94微米的直径制备的微球在模拟胃液中保留了维生素，并在模拟肠液中释放了维生素。通过精心的工艺设计，有望开发出具有作为生物活性化合物口服递送载体潜力的颗粒微球。
