Hagihara Masaki, Hasegawa Tetsuya, Tanabe Yukiko, Sato Shin-ichi, Yoshikawa Susumu, Ohkubo Katsutoshi, Morii Takashi
Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011, Japan.
Nucleic Acids Symp Ser (Oxf). 2004(48):33-4. doi: 10.1093/nass/48.1.33.
Here we report a simple method to convert synthetic receptors to fluorescent sensors. An RNA-peptide complex (ribonucleopeptide) with a known three-dimensional structure is used as a framework of the receptor. Artificial ribonucleopeptide sensors were created with a combination of in vitro selection method and successive modification of the peptide with a fluorophore. A ribonucleopeptide complex of the fluorophore labeled peptide showed a remarkable fluorescence emission change upon binding cognate ligands.
在此，我们报告一种将合成受体转化为荧光传感器的简单方法。具有已知三维结构的RNA-肽复合物（核糖核肽）被用作受体的框架。通过体外筛选方法与用荧光团对肽进行连续修饰相结合，创建了人工核糖核肽传感器。荧光团标记肽的核糖核肽复合物在结合同源配体时显示出显著的荧光发射变化。
