Kamiya Hiroyuki, Satou Kazuya, Hori Mika, Iida Emiko, Ishiguro Chieko, Harashima Hideyoshi
Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan.
Nucleic Acids Symp Ser (Oxf). 2004(48):271-2. doi: 10.1093/nass/48.1.271.
Oxidized deoxyribonucleotides, 2-hydroxydeoxyadenosine 5'-triphosphate (2-OH-dATP) and 8-hydroxydeoxyguanosine 5'-triphosphate (8-OH-dGTP), were introduced into Escherichia coli strains deficient in DNA polymerase IV (a Y-family DNA polymerase encoded in the dinB gene), and the MutT and Orfl35 proteins to examine their in vivo roles in mutagenesis elicited by 2-OH-dATP and 8-OH-dGTP. 2-OH-dATP elicited mutations less efficiently in the dinB- strain than in the wild type strain, suggesting involvement of DNA polymerase IV in 2-OH-dATP-induced mutations. 8-OH-dGTP and 2-OH-dATP elicited mutations more efficiently in mutT- and orfl35- strains, respectively, than those in their isogenic mutT+ and orfl35+ strains. These results indicate that these proteins play important roles in mutagenesis induced by 2-OH-dATP and 8-OH-dGTP in vivo.
将氧化脱氧核糖核苷酸2-羟基脱氧腺苷5'-三磷酸（2-OH-dATP）和8-羟基脱氧鸟苷5'-三磷酸（8-OH-dGTP）导入缺乏DNA聚合酶IV（由dinB基因编码的Y家族DNA聚合酶）以及MutT和Orfl35蛋白的大肠杆菌菌株中，以研究它们在由2-OH-dATP和8-OH-dGTP引发的诱变中的体内作用。与野生型菌株相比，2-OH-dATP在dinB-菌株中诱发突变的效率较低，这表明DNA聚合酶IV参与了2-OH-dATP诱导的突变。与同基因的mutT+和orfl35+菌株相比，8-OH-dGTP和2-OH-dATP分别在mutT-和orfl35-菌株中诱发突变的效率更高。这些结果表明，这些蛋白质在体内由2-OH-dATP和8-OH-dGTP诱导的诱变中起重要作用。
