Aso Mariko, Kaneko Toshiyuki, Mirc John Walter, Koga Noboru, Suemune Hiroshi
Graduate School of Pharmaceutical Sciences, Kyushu University, Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
Nucleic Acids Symp Ser (Oxf). 2005(49):3-4. doi: 10.1093/nass/49.1.3.
Oligodeoxynucleotide (ODN; 1) containing 2-N-tert-butylaminoxyladenosine (2) was synthesized by the conventional phosphoramidite method. The thermal stability and structure of the duplex of ODN 1 and the complementary strand were elucidated by UV-melting temperature measurements and CD experiments. In phosphate buffer, ODN 1 showed a three line EPR spectrum. Upon duplex formation, the EPR spectrum of 1 changed which indicates that EPR spectra of 1 can provide information on structures of ODN.
通过传统的亚磷酰胺法合成了含有2-N-叔丁基氨基氧基腺苷（2）的寡脱氧核苷酸（ODN；1）。通过紫外熔解温度测量和圆二色性实验阐明了ODN 1与互补链双链体的热稳定性和结构。在磷酸盐缓冲液中，ODN 1显示出三线EPR光谱。双链体形成后，1的EPR光谱发生变化，这表明1的EPR光谱可以提供有关ODN结构的信息。
