Kawanaka Toshihide, Shimizu Mamoru, Saigo Kazuhiko, Wada Takeshi
Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Bioscience building 702, Kashiwa, Chiba 277-8562, Japan.
Nucleic Acids Symp Ser (Oxf). 2005(49):27-8. doi: 10.1093/nass/49.1.27.
Recently, we have developed a novel reaction for the transformation of boranophosphate diesters to the corresponding H-phosphonate diesters in the presence of trityl cation under acidic conditions. In this study, DNA and backbone-modified DNA analogs were synthesized in good yields upon applying this reaction. We report the transformation of boranophosphate DNAs, fully protected with 2-azidomethylbenzoyl groups, to various backbone-modified DNA analogs via the H-phosphonate intermediates in solution and on a solid support.
最近，我们开发了一种新型反应，即在酸性条件下，在三苯甲基阳离子存在的情况下，将硼酸磷酸二酯转化为相应的H-膦酸二酯。在本研究中，应用该反应以良好的产率合成了DNA和骨架修饰的DNA类似物。我们报道了在溶液中和在固体支持物上，通过H-膦酸酯中间体将用2-叠氮甲基苯甲酰基完全保护的硼酸磷酸酯DNA转化为各种骨架修饰的DNA类似物。
