Hoshika Shuichi, Minakawa Noriaki, Matsuda Akira
Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan.
Nucleic Acids Symp Ser (Oxf). 2005(49):77-8. doi: 10.1093/nass/49.1.77.
Short interfering RNAs (siRNAs) variously modified with 4'-thioribonucleosides against the photinus luciferase gene and the renilla luciferase gene were tested for their induction of the RNA interference (RNAi) activity in cultured NIH/3T3 cells. Results indicated that modifications with 4'-thioribonucleosides on siRNA against the photinus luciferase were as potent as natural siRNA. On the other hand, modifications with 4'-thioribonucleosides on natural siRNA against the renilla luciferase improved their RNAi activity. These results suggest that 4'-thioribonucleosides might be potentially useful in the development of novel and effective chemically modified siRNAs.
针对萤火虫荧光素酶基因和海肾荧光素酶基因，用4'-硫代核糖核苷进行了各种修饰的小干扰RNA（siRNA），在培养的NIH/3T3细胞中测试了它们诱导RNA干扰（RNAi）活性的能力。结果表明，针对萤火虫荧光素酶的siRNA上用4'-硫代核糖核苷进行修饰与天然siRNA一样有效。另一方面，针对海肾荧光素酶的天然siRNA上用4'-硫代核糖核苷进行修饰提高了它们的RNAi活性。这些结果表明，4'-硫代核糖核苷可能在新型有效化学修饰siRNA的开发中具有潜在用途。
