Kuwahara Masayasu, Ohsawa Kazuomi, Kasamatsu Toshiyuki, Shoji Atsushi, Sawai Hiroaki, Ozaki Hiroaki
Department of Applied Chemistry, Fuclty of Engneering, Gunma University, 1-5-1 Tenjin-chou, Kiryu, Gunma 376-8515, Japan.
Nucleic Acids Symp Ser (Oxf). 2005(49):81-2. doi: 10.1093/nass/49.1.81.
A binder that is specific for a target will be applicable to detection of a variety of molecules such as bioactive substances, environmental contaminants and so on. Nucleic acid aptamers obtained by in vitro selection method are interesting and promising binders. To enhance the ability of nucleic acid aptamers, we prepared a combinatorial modified DNA library containing arginyl uracil base by using arginyl dUTP, then attempted screening of glutamic acid-binding aptamers from the library by in vitro selection method.
对靶标具有特异性的结合剂可应用于多种分子的检测，如生物活性物质、环境污染物等。通过体外筛选方法获得的核酸适体是有趣且有前景的结合剂。为了增强核酸适体的能力，我们使用精氨酰dUTP制备了一个包含精氨酰尿嘧啶碱基的组合修饰DNA文库，然后尝试通过体外筛选方法从该文库中筛选谷氨酸结合适体。
