Hasiba Waka, Xu Yan, Sugiyama Hiroshi
Graduate School of Science, Kyoto University, Kitashirakawa-Oiwakecho, Sakyo, Kyoto, 606-8502, Japan.
Nucleic Acids Symp Ser (Oxf). 2005(49):179-80. doi: 10.1093/nass/49.1.179.
DNA C1' radicals formed by hydrogen atom abstraction from the C1' position of the sugar backbone lead to the production of 2'-deoxyribonolactone as a DNA damage lesion. Although DNA C1' radicals may be involved in a variety of DNA damage processes, there is very little information on the molecular basis of DNA structure dependence in the reactions of C1' radicals. Here, we describe a detailed study of the reactivity of 2'-deoxyuridin-1'-yl radicals in single- and double-stranded B-form DNA and G-quartets in the formation of 2'-deoxyribonolactone, using the unnatural precursor 1'-pivaloyl-2'-deoxyuridine (1).
从糖骨架的C1'位置夺取氢原子形成的DNA C1'自由基会导致产生2'-脱氧核糖内酯这种DNA损伤病变。尽管DNA C1'自由基可能参与多种DNA损伤过程，但关于C1'自由基反应中DNA结构依赖性的分子基础的信息却非常少。在此，我们使用非天然前体1'-新戊酰基-2'-脱氧尿苷（1），详细研究了单链和双链B型DNA以及G-四联体中2'-脱氧尿苷-1'-基自由基在形成2'-脱氧核糖内酯过程中的反应活性。
