Fujii Shiho, Obika Satoshi, Imanishi Takeshi
Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Nucleic Acids Symp Ser (Oxf). 2005(49):211-2. doi: 10.1093/nass/49.1.211.
Molecular beacons (MBs) are stem-loop hairpin oligonucleotide probes with an internally quenched fluorophore. These probes recognize their targets with higher specificity than conventional linear probes. To further enhance sequence-specificity of MBs, we have designed and synthesized MBs having 2',4'-BNA modification. Thermodynamic analysis revealed that the MBs with the 2',4'-BNA-modified stem region have high sequence-specificity. In addition, the 2',4'-BNA modification in the loop region was also found to be efficient for discrimination of one base mismatch.
分子信标（MBs）是一种带有内部猝灭荧光团的茎环发夹寡核苷酸探针。这些探针识别靶标的特异性高于传统线性探针。为了进一步提高分子信标的序列特异性，我们设计并合成了具有2'，4'-双环核酸（BNA）修饰的分子信标。热力学分析表明，茎区具有2'，4'-BNA修饰的分子信标具有高序列特异性。此外，还发现环区的2'，4'-BNA修饰对于区分单碱基错配也是有效的。
