Nomura Makoto, Hagihara Shinya, Goto Yuki, Nakatani Kazuhiko, Kojima Chojiro
Graduate School of Biological Science, Nara Institute of Science and Technology, Nara 630-0101, Japan.
Nucleic Acids Symp Ser (Oxf). 2005(49):213-4. doi: 10.1093/nass/49.1.213.
Naphthyridine-dimer (ND) specifically recognizes G.G mismatch DNA (Figure 1). However, its detailed recognition mechanism is not clear. Here a DNA oligomer d(CTAACGGAATG)/d(CATTCGGTTAA) complexed with ND was studied by NMR. The stoichiometry of DNA to ND was determined to be 1:2 at NMR concentration (2.5 mM). Proton resonances were completely assigned including H5' and H5'' using 1H-1H and 1H-13C 2D spectra of the complex. These spectra showed that four naphthyridine rings are staked in the helix and form hydrogen bonds with the four G residues in CGG/CGG region. These results indicate ND can specifically recognize the CGG/CGG sequence.
萘啶二聚体（ND）能特异性识别G·G错配DNA（图1）。然而，其详细的识别机制尚不清楚。在此，通过核磁共振（NMR）研究了与ND复合的DNA寡聚物d(CTAACGGAATG)/d(CATTCGGTTAA)。在NMR浓度（2.5 mM）下，确定DNA与ND的化学计量比为1:2。利用该复合物的1H-1H和1H-13C二维光谱，完全归属了包括H5'和H5''在内的质子共振。这些光谱表明，四个萘啶环堆积在螺旋中，并与CGG/CGG区域的四个G残基形成氢键。这些结果表明ND能特异性识别CGG/CGG序列。
