嗜热古菌火球菌K1中甘氨酰-tRNA合成酶对甘氨酸tRNA的识别位点。
Recognition sites of glycine tRNA for glycyl-tRNA synthetase from hyperthermophilic archaeon, Aeropyrum pernix K1.
作者信息
Okamoto Koji, Kuno Atsushi, Hasegawa Tsunemi
机构信息
Graduate School of Science and Technology, Yamagata University, Yamagata 990-8560, Japan.
出版信息
Nucleic Acids Symp Ser (Oxf). 2005(49):299-300. doi: 10.1093/nass/49.1.299.
To elucidate the tRNA recognition sites of glycine tRNA from an extreme thermophilic and aerobic archaeon, Aeropyrum pernix K1, we examined glycylation activities using in vitro mutant glycine tRNA transcripts and recombinant A. pernix glycyl-tRNA synthetase. The recognition nucleotides were determined to be C35 and C36 of anticodon, C2-G71 and G3-C70 base-pairs of acceptor stem. However, discriminator base A73 was not recognized by glycyl-tRNA synthetase.
为了阐明来自极端嗜热需氧古菌——火球菌K1（Aeropyrum pernix K1）的甘氨酸tRNA的tRNA识别位点，我们使用体外突变的甘氨酸tRNA转录本和重组火球菌甘氨酰-tRNA合成酶检测了甘氨酰化活性。确定识别核苷酸为反密码子的C35和C36、受体茎的C2-G71和G3-C70碱基对。然而，鉴别碱基A73未被甘氨酰-tRNA合成酶识别。
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