杆状病毒介导的shRNA表达对丙型肝炎病毒RNA复制的抑制作用。

Suppression of HCV RNA replication by baculovirus-mediated shRNA expression.

作者信息

Suzuki Hitoshi, Kaneko Hiroyasu, Tamai Nobushige, Miyano-Kurosaki Naoko, Hashimoto Kahoko, Shimotohno Kunitada, Takaku Hiroshi

机构信息

Department of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2005(49):339-40. doi: 10.1093/nass/49.1.339.

DOI:10.1093/nass/49.1.339

PMID:17150772

Abstract

We describe a HCV core-short-hairpin RNA (shRNA) expressed from a human U6 Pol II promoter, which synergistically enhanced the inhibition of HCV replication in HCV replicon. We also describe the construction of recombinant baculovirus expressing shRNAs of the HCV core gene under the control of the U6 promoter. The anti-HCV shRNAs may provide a practical basis for applying shRNA-based gene therapy to the treatment of HCV.

摘要

我们描述了一种从人U6 RNA聚合酶II启动子表达的丙型肝炎病毒（HCV）核心短发夹RNA（shRNA），其可协同增强对HCV复制子中HCV复制的抑制作用。我们还描述了在U6启动子控制下表达HCV核心基因shRNAs的重组杆状病毒的构建。抗HCV shRNAs可为基于shRNA的基因治疗应用于HCV治疗提供实际依据。