一种新型DNA沟结合剂的双荧光光化学评估

Photochemical evaluation of dual fluorescence of a novel DNA groove binder.

作者信息

Tanaka Kazuo, Tainaka Kazuki, Fujishima Shohei, Okamoto Akimitsu

机构信息

Frontier Research System, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2006(50):137-8. doi: 10.1093/nass/nrl068.

DOI:10.1093/nass/nrl068

PMID:17150855

Abstract

We synthesized the fluorophore-tethered oligopeptide showing a dual fluorescence in water, and the ratiometric fluorescence change of the modified oligopeptide was induced by the binding to DNA. The equilibrium of the modified oligopeptide between two excited states, a locally excited (LE) state and an intramolecular charge transfer (ICT) state, was controlled depending to DNA structures.

摘要

我们合成了在水中呈现双荧光的荧光团连接寡肽，并且修饰后的寡肽的比率荧光变化是由与DNA结合诱导产生的。修饰后的寡肽在局部激发（LE）态和分子内电荷转移（ICT）态这两个激发态之间的平衡根据DNA结构而受到调控。