Casado Flávia, Bonvicino Cibele R, Seuánez Héctor N
Genetics Division, Instituto Nacional de Câncer, Rua André Cavalcanti, 37, 4th floor, 20231-050, Rio de Janeiro, RJ, Brazil.
J Hered. 2007 Jan-Feb;98(1):88-92. doi: 10.1093/jhered/esl054. Epub 2006 Dec 5.
A phylogeographic study of Callicebus lugens was carried out based on cytochrome b DNA sequence data. Here, we report, for the first time, the distribution of C. lugens south of the Rio Negro, in Barcelos municipality (Amazonas State, Brazil), indicating that this river is not the southern boundary of the distribution of this species as previously proposed. Specimens from the north and south banks showed the same diploid number (2n = 16), while phylogenetic reconstructions based on maximum parsimony, distance, and maximum likelihood analyses grouped all specimens in a strongly supported clade comprising 2 separate lineages, in coincidence with their geographic distribution along riverbanks. Median-joining analysis showed a similar separation, with 22 transitions between the 2 groups, whereas time of divergence estimates indicated that the splitting of the C. lugens lineages occurred some 2.2 million years before present. Conservation strategies should take into consideration that this species might be sympatric with Callicebus torquatus at the south bank of Rio Negro.
基于细胞色素b DNA序列数据对白领伶猴进行了系统地理学研究。在此，我们首次报告了白领伶猴在巴西亚马逊州巴塞罗斯市黑河以南的分布情况，这表明这条河并非如先前所提出的是该物种分布的南部边界。来自北岸和南岸的样本显示出相同的二倍体数目（2n = 16），而基于最大简约法、距离法和最大似然法分析的系统发育重建将所有样本归为一个得到有力支持的分支，该分支包含两个独立的谱系，这与它们沿河岸的地理分布情况相符。中介邻接分析显示出类似的分离情况，两组之间有22个转换，而分歧时间估计表明白领伶猴谱系的分裂发生在距今约220万年前。保护策略应考虑到该物种可能与黑河以南岸的白领伶猴同域分布。
