Wiegel D, Richter O, Arnhold J, Arnold K
University of Leipzig, School of Medicine, Institute of Biophysics, FRG.
Biomed Biochim Acta. 1991;50(2):207-12.
Aqueous two-phase systems containing polyethylene glycol (PEG) and dextran as phase forming polymers were used for the partition of unmodified and hypochlorite modified lipoproteins. Low density lipoprotein (LDL) was separated from high density lipoprotein (HDL) by sequential ultracentrifugation from human plasma. In agreement with the higher electrophoretic mobility, high density lipoprotein shows a higher value of the partition coefficient in contrast to low density lipoprotein. An increase in the concentration of chloride ions reduces the enrichment of lipoprotein in the top phase and favours the accumulation of aggregated material at the interface. The partition coefficient strongly depends on the age of the lipoprotein sample. Differences in the value of the partition coefficient could be obtained for the lipoprotein fractions HDL-2 and HDL-3. Hypochlorite modified LDL shows higher values of the partition coefficient due to the higher negative charge of the modified lipoprotein particle.
以聚乙二醇（PEG）和葡聚糖作为成相聚合物的双水相系统用于未修饰和次氯酸盐修饰的脂蛋白的分配。通过从人血浆中进行连续超速离心，将低密度脂蛋白（LDL）与高密度脂蛋白（HDL）分离。与较高的电泳迁移率一致，高密度脂蛋白与低密度脂蛋白相比显示出更高的分配系数值。氯离子浓度的增加会降低脂蛋白在上层相中的富集，并有利于聚集物质在界面处的积累。分配系数强烈依赖于脂蛋白样品的保存时间。对于HDL-2和HDL-3脂蛋白组分，可以获得分配系数值的差异。次氯酸盐修饰的LDL由于修饰的脂蛋白颗粒具有更高的负电荷而显示出更高的分配系数值。
