采用聚乙二醇6000和硫酸葡聚糖联合沉淀法分离血浆高密度脂蛋白亚类。
Separation of plasma high-density lipoprotein subclasses by a combined precipitation method using polyethylene glycol 6000 and dextran sulphate.
作者信息
Lundberg B, Högström S, Pietiläinen P, Suominen M, Viikari J
出版信息
Scand J Clin Lab Invest. 1984 Jun;44(4):305-9. doi: 10.3109/00365518409083812.
A fast and cheap precipitation method for the separation of HDL subclasses been evaluated. It starts with precipitation of apo-B containing lipoproteins with PEG-6000 after which HDL2 is precipitated with dextran sulphate-Mg2+. A concentration of 0.72 g/l dextran sulphate was found to be suitable for the separation of HDL subclasses. HDL subclasses obtained by precipitation gave proper density profiles at density gradient centrifugations. The correlations between results obtained by this method and by sequential and density gradient centrifugation are good, r = 0.99 and 0.72, respectively.
已评估了一种用于分离高密度脂蛋白（HDL）亚类的快速且廉价的沉淀方法。该方法首先用聚乙二醇-6000（PEG-6000）沉淀含载脂蛋白B的脂蛋白，之后用硫酸葡聚糖-Mg2+沉淀HDL2。发现0.72 g/l的硫酸葡聚糖浓度适用于HDL亚类的分离。通过沉淀获得的HDL亚类在密度梯度离心时呈现出合适的密度分布。该方法与连续离心法和密度梯度离心法所得结果之间的相关性良好，分别为r = 0.99和0.72。
Zotero 插件