[紫杉醇诱导MCF-7人乳腺癌细胞凋亡的蛋白质组学分析]
[Proteomic analysis of paclitaxel-induced apoptosis in MCF-7 human breast carcinoma cells].
作者信息
Wu Feng, Wang Qing-Ming, Fan Guo-Cai, Chen Ji-Zhong, Chen Hui-Peng
机构信息
Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
出版信息
Zhonghua Zhong Liu Za Zhi. 2006 Jun;28(6):418-21.
OBJECTIVE
To investigate the mechanism of paclitaxel-induced apoptosis in MCF-7 human breast carcinoma cells.
METHODS
In this study, the proteins extracted from paclitaxel-induced apoptotic MCF-7 cells were analyzed by 2-dimentional gel electrophoresis (2-DE), and compared with those from untreated MCF-7 cells. The differential proteins were identified by mass spectrometry.
RESULTS
At 24 hour after paclitaxel (100 nmol/L) treatment, MCF-7 cells were collected and extracted the whole proteins. Seventeen up-regulated or down-regulated proteins were found by analysis of the differential proteomic 2-DE map. Six of them were identified by mass spectrometry. They were enolase 1, chloride intracellular channel 1, keratin 8, ribosomal protein S12, galectin-1 and histidine triad nucleotide binding protein, respectively.
CONCLUSION
We effectively found the changed proteins in the process of paclitaxel-induced apoptosis in MCF-7 human breast carcinoma cells by proteomic techniques. These up-regulated or down-regulated proteins are important molecules for our further research about the mechanism of paclitaxel-induced apoptosis.
目的
探讨紫杉醇诱导MCF-7人乳腺癌细胞凋亡的机制。
方法
本研究采用二维凝胶电泳（2-DE）分析紫杉醇诱导凋亡的MCF-7细胞所提取的蛋白质，并与未处理的MCF-7细胞的蛋白质进行比较。通过质谱鉴定差异蛋白质。
结果
用1〇〇nmol/L紫杉醇处理24小时后，收集MCF-7细胞并提取总蛋白。通过差异蛋白质组2-DE图谱分析发现17种上调或下调的蛋白质。其中6种通过质谱鉴定，分别为烯醇化酶1、细胞内氯离子通道1、角蛋白8、核糖体蛋白S12、半乳糖凝集素-1和组氨酸三联体核苷酸结合蛋白。
结论
我们通过蛋白质组学技术有效地发现了紫杉醇诱导MCF-7人乳腺癌细胞凋亡过程中变化的蛋白质。这些上调或下调的蛋白质是我们进一步研究紫杉醇诱导凋亡机制的重要分子。
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