Gasser R B, Lightowlers M W, Rickard M D
University of Melbourne, Veterinary Clinical Centre, Werribee, Victoria, Australia.
Res Vet Sci. 1991 May;50(3):340-5. doi: 10.1016/0034-5288(91)90135-b.
Proteins present in oncospheres and on the surface of living protoscoleces of Echinococcus granulosus were radioiodinated by the lodogen technique and immunoprecipitated with sera from dogs with E granulosus infection and several categories of control sera. Analysis of immunoprecipitates was performed using sodium dodecyl-sulphate polyacrylamide gel electrophoresis to identify antigenic protein components specific for E granulosus. Sera from dogs with E granulosus infection identified antigenic proteins of around Mr 37,000, 30,000 or 22,000 in oncospheres, and proteins of around Mr 70,000, 43,000, 36,000, 27,000 (triplet), 20,000 or 14,000 on the surface of protoscoleces. These antigens appear to be both species- and stage-specific and may be useful for serological discrimination between 'current' and 'recent past' prepatent and patent E granulosus infections in dogs.
利用碘标记技术对细粒棘球绦虫原头蚴和六钩蚴中的蛋白质进行放射性碘化，并使用细粒棘球绦虫感染犬的血清以及几类对照血清进行免疫沉淀。采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳对免疫沉淀物进行分析，以鉴定细粒棘球绦虫特有的抗原性蛋白质成分。细粒棘球绦虫感染犬的血清鉴定出六钩蚴中约37,000、30,000或22,000相对分子质量的抗原性蛋白质，以及原头蚴表面约70,000、43,000、36,000、27,000（三联体）、20,000或14,000相对分子质量的蛋白质。这些抗原似乎具有种属和阶段特异性，可能有助于对犬类中“当前”和“近期过去”的细粒棘球绦虫感染前和感染期进行血清学鉴别。
