Bender P K
Department of Biochemistry and Nutrition, Virginia Polytechnic Institute, Blacksburg 24061.
Biochem Biophys Res Commun. 1991 Aug 30;179(1):707-12. doi: 10.1016/0006-291x(91)91430-k.
In the adult I/LnJ mouse skeletal muscle, phosphorylase kinase activity is 0.2% of that in normal. This deficiency results from a paucity of mRNA's for the phosphorylase kinase regulatory subunit- alpha and its isoform alpha'. However, in the I/LnJ neonatal skeletal muscle phosphorylase kinase activity is 20-25% of that in normal. During the first two months of development this activity decreases while in normal tissue it increases. The developmental differences in the magnitude of the I/LnJ deficiency indicate the possibility of stage specific mechanisms regulating the accumulation of alpha/alpha' mRNAs. To investigate this possibility, the abundance of alpha/alpha' mRNAs and of the catalytic subunit, gamma, mRNAs were compared by Northern Blot analysis. The results demonstrate that neonatal and adult I/LnJ skeletal muscle have a similar paucity of alpha/alpha' mRNAs whereas accumulation of gamma mRNAs is not significantly different from normal.
在成年I/LnJ小鼠骨骼肌中，磷酸化酶激酶活性仅为正常水平的0.2%。这种活性缺乏是由于磷酸化酶激酶调节亚基α及其异构体α'的mRNA数量不足所致。然而，在I/LnJ新生小鼠骨骼肌中，磷酸化酶激酶活性为正常水平的20%-25%。在发育的前两个月，该活性下降，而正常组织中的活性则升高。I/LnJ小鼠活性缺乏程度的发育差异表明，可能存在阶段特异性机制调节α/α' mRNA的积累。为了研究这种可能性，通过Northern印迹分析比较了α/α' mRNA和催化亚基γ mRNA的丰度。结果表明，新生和成年I/LnJ小鼠骨骼肌中α/α' mRNA的数量同样稀少，而γ mRNA的积累与正常情况相比无显著差异。
