Bathgate R, Maxwell W M C, Evans G
Centre for Advanced Technologies in Animal Genetics and Reproduction (ReproGen), Faculty of Veterinary Science, The University of Sydney, NSW 2006, Australia.
Theriogenology. 2007 Mar 1;67(4):886-92. doi: 10.1016/j.theriogenology.2006.09.043. Epub 2006 Dec 6.
Cryopreservation of boar sperm compromises fertility after thawing by reducing sperm longevity and inducing acrosome reaction-like changes. In an attempt to improve the post-thaw motility and acrosome integrity of boar sperm, semen was frozen using a modified Westendorf method in which the medium was supplemented with either platelet-activating factor (PAF) or a recombinant platelet-activating factor:acetylhydrolase (PAF:AH; Pafase) before or after freezing. Platelet-activating factor is a phospholipid that is present in boar semen and PAF:AH is the naturally occurring enzyme that converts PAF to biologically inactive Lyso-PAF. Addition of PAF to the cryopreservation medium improved post-thaw motility immediately after thawing and after 3h incubation at 37 degrees C (60.0+/-0.0% and 25.0+/-2.9%; mean+/-S.E.M.) compared to the control sperm (41.7+/-1.7% and 10.0+/-2.9%; P<0.05). Acrosome integrity was higher immediately after thawing and after 3 and 6h incubation at 37 degrees C when sperm were frozen in the presence of Pafase (55.7+/-3.2%, 45.7+/-3.7% and 23.0+/-3.1%), compared to the control sperm (42.7+/-1.5%, 25.7+/-5.7% and 12.3+/-2.7%) and sperm frozen in the presence of PAF (33.0+/-3.7%, 26.3+/-2.2% and 11.7+/-0.3%; P<0.05). Addition of PAF to sperm after thawing improved motility immediately post-thaw (41.6+/-2.6%), compared with addition of Pafase (23.3+/-2.2%) or the control sperm with no supplementation of the medium (26.7+/-2.2%; P<0.05). However, this beneficial effect was lost by 3h post-thaw. Supplementation of boar semen cryopreservation medium with PAF and Pafase appeared to have beneficial effects on the in vitro quality of the sperm post-thaw.
公猪精子的冷冻保存会通过降低精子寿命和引发类似顶体反应的变化而损害解冻后的生育能力。为了提高公猪精子解冻后的活力和顶体完整性，采用改良的韦斯顿多夫方法冷冻精液，即在冷冻前或冷冻后向培养基中添加血小板活化因子（PAF）或重组血小板活化因子：乙酰水解酶（PAF:AH；Pafase）。血小板活化因子是一种存在于公猪精液中的磷脂，而PAF:AH是将PAF转化为生物活性较低的溶血PAF的天然存在的酶。与对照精子（41.7±1.7%和10.0±2.9%；P<0.05）相比，在冷冻保存培养基中添加PAF可使解冻后立即以及在37℃孵育3小时后的解冻后活力提高（60.0±0.0%和25.0±2.9%；平均值±标准误）。当精子在Pafase存在下冷冻时，解冻后立即以及在37℃孵育3小时和6小时后的顶体完整性更高（55.7±3.2%、45.7±3.7%和23.0±3.1%），与对照精子（42.7±1.5%、25.7±5.7%和12.3±2.7%）以及在PAF存在下冷冻的精子（33.0±3.7%、26.3±2.2%和11.7±0.3%；P<0.05）相比。解冻后向精子中添加PAF可使解冻后立即的活力提高（41.6±2.6%），与添加Pafase（23.3±2.2%）或未补充培养基的对照精子（26.7±2.2%；P<0.05）相比。然而，这种有益效果在解冻后3小时消失。在公猪精液冷冻保存培养基中添加PAF和Pafase似乎对解冻后精子的体外质量有有益影响。
