Möller Asja, Truyen Uwe, Roesler Uwe
Institute of Animal Hygiene and Veterinary Public Health, Faculty of Veterinary Medicine, University of Leipzig, An den Tierklinken 1, D-04103 Leipzig, Germany.
Vet Microbiol. 2007 Mar 10;120(3-4):370-4. doi: 10.1016/j.vetmic.2006.10.039. Epub 2006 Nov 10.
In order to clarify the epidemiology of bovine protothecal mastitis, 30 Prototheca zopfii mastitis isolates were genetically investigated. Based on the 18S rDNA, which allows a differentiation of the former species P. zopfii in two distinct P. zopfii genotypes and Prototheca blaschkeae sp. nov., newly developed genotype-specific PCR-assays as well as RFLP-assays were applied. All mastitis isolates investigated could be assigned to P. zopfii genotype 2 suggesting that this genotype is the aetiological agent of bovine Prototheca mastitis.
为了阐明牛原虫性乳腺炎的流行病学，对30株威克原虫性乳腺炎分离株进行了基因研究。基于18S rDNA，其可将原威克原虫物种分为两种不同的威克原虫基因型和新发现的布拉施克原虫物种。新开发的基因型特异性PCR检测方法以及限制性片段长度多态性（RFLP）检测方法被应用。所有被研究的乳腺炎分离株都可归为威克原虫基因型2，这表明该基因型是牛原虫性乳腺炎的病原体。
