Dinallo R M, Davidson W C, Hansen G E
Department of Analytical Sciences, Boehringer Ingelheim Pharmaceuticals Inc., Ridgefield, Connecticut 06877.
Biol Mass Spectrom. 1991 May;20(5):268-74. doi: 10.1002/bms.1200200506.
Characterization of unknown synthetic by-products from a test synthesis of the experimental inhibitor of HIV-1 reverse transcriptase, BI-RG-587, is presented. Seven impurities were separated and characterized by thermospray liquid chromatography/mass spectrometry using low-resolution, high-resolution, and B/E linked scanning methods. The high-resolution mass measurements which were obtained were accurate to within 10 ppm of structures proposed for synthetic by-products present at the 0.1% level. Linked scans produced daughter ion spectra for each impurity to further aid in the characterization.
本文介绍了对HIV-1逆转录酶实验性抑制剂BI-RG-587测试合成中未知合成副产物的表征。通过热喷雾液相色谱/质谱联用，采用低分辨率、高分辨率和B/E联动扫描方法，分离并表征了七种杂质。所获得的高分辨率质谱测量结果与所提出的在0.1%水平存在的合成副产物结构的误差在10 ppm以内。联动扫描为每种杂质产生子离子光谱，以进一步辅助表征。
