Glaser R W, Volk H D, Liebenthal C, Jahn S, Grunow R
Department of Medical Immunology, Medical School (Charité), Humboldt University Berlin, GDR.
Hum Antibodies Hybridomas. 1990;1(2):111-4.
Human lymphocytes from peripheral blood (MNC) were separated on magnetic beads for the presence of different surface markers. Cells from positive and negative fractions were successfully immortalized by electrofusion with the heteromyeloma line CB-Fu2. B cells, which were separated on anti-CD 19 coated beads, could be immortalized at a rate between 10(-5) and 10(-4) even if the fusion was conducted with just a few hundred thousand cells. Comparison of the frequency of Ig-positive hybridomas in B cell, T cell, and unseparated MNC fusions indicated that also non-B cells may give rise to HAT resistant hybridoma clones, although the fusion frequency was low.
利用磁珠分离人外周血淋巴细胞（MNC）以检测不同表面标志物的存在。通过与异骨髓瘤细胞系CB - Fu2进行电融合，成功使阳性和阴性组分的细胞永生化。在抗CD19包被的磁珠上分离得到的B细胞，即使仅用几十万细胞进行融合，也能以10⁻⁵至10⁻⁴的比率实现永生化。对B细胞、T细胞和未分离的MNC融合中Ig阳性杂交瘤频率的比较表明，尽管融合频率较低，但非B细胞也可能产生对HAT有抗性的杂交瘤克隆。
