来自CaMKK的序列反向肽以可逆的Ca2+依赖性方式与钙调蛋白结合。

Sequence reversed peptide from CaMKK binds to calmodulin in reversible Ca2+ -dependent manner.

作者信息

Li Isaac T S, Ranjith K R, Truong Kevin

机构信息

Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, Ont., Canada M5S 3G9.

出版信息

Biochem Biophys Res Commun. 2007 Jan 26;352(4):932-5. doi: 10.1016/j.bbrc.2006.11.123. Epub 2006 Dec 4.

DOI:10.1016/j.bbrc.2006.11.123

PMID:17157809

Abstract

Calmodulin (CaM) is a highly versatile Ca(2+) signaling transducer known to regulate over a hundred proteins. In this paper, we further demonstrate the versatility of CaM binding by showing that it binds to a synthetic peptide (revCKKp) made by reversing the amino acid sequence of the CaM-binding peptide (CKKp) from CaM-dependent protein kinase kinase (CaMKK) (residues 438-463). Sequence comparison between revCKKp and other CaM-binding peptides (CBPs) from the CaM target databank showed that revCKKp does not resemble any existing classes of CBPs, except CKKp [M. Zhang, T. Yuan, Molecular mechanisms of calmodulin's functional versatility, Biochem. Cell Biol. 76 (1998) 313-323; S.W. Vetter, E. Leclerc, Novel aspects of calmodulin target recognition and activation, Eur. J. Biochem. 270 (2003) 404-414]. Furthermore, computational modeling showed that revCKKp could bind CaM in a similar manner to CKKp. Lastly, we experimentally showed that our synthetic revCKKp binds to CaM in a reversible Ca(2+)-dependent manner.

摘要

钙调蛋白（CaM）是一种高度通用的Ca(2+)信号转导蛋白，已知可调节一百多种蛋白质。在本文中，我们通过证明它与一种合成肽（revCKKp）结合，进一步展示了CaM结合的通用性，该合成肽是通过反转来自钙调蛋白依赖性蛋白激酶激酶（CaMKK）（第438 - 463位氨基酸残基）的钙调蛋白结合肽（CKKp）的氨基酸序列而制成的。revCKKp与钙调蛋白靶标数据库中的其他钙调蛋白结合肽（CBP）之间的序列比较表明，除了CKKp外，revCKKp与任何现有的CBP类别都不相似[M. Zhang, T. Yuan, 钙调蛋白功能通用性的分子机制，生物化学与细胞生物学76 (1998) 313 - 323；S.W. Vetter, E. Leclerc, 钙调蛋白靶标识别和激活的新方面，欧洲生物化学杂志270 (2003) 404 - 414]。此外，计算模型表明revCKKp可以与CKKp以类似的方式结合钙调蛋白。最后，我们通过实验表明，我们合成的revCKKp以可逆的Ca(2+)依赖性方式结合钙调蛋白。