Nosál' Radomir, Drábiková Katarína, Jancinová Viera, Macicková Tatiana, Pecivová Jana, Holománová Dagmar
Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava, Slovakia.
Neuro Endocrinol Lett. 2006 Dec;27 Suppl 2:148-51.
To study the effect of H1-antihistamines dithiaden (Dit) and loratadine (Lor) and compare it with that of histamine (His) on phorbolmyristate acetate (PMA) stimulated chemiluminescence (CL) of whole blood, isolated neutrophils, release of myeloperoxidase (MPO), and on superoxide (SO) generation.
Luminol- and isoluminol-enhanced CL was applied for measuring the oxidative burst, spectrophotometry was used for determination of MPO (o-dianisidine) and SO generation (superoxide dismutase inhibition of cytochrome c).
Dit and Lor dose-dependently inhibited CL of whole blood and significantly decreased oxidative burst both at the extra- and intracellular sites of neutrophils. Release of MPO was decreased with both drugs tested in 10-times lower concentrations than was SO inhibition. Histamine (His) was much less effective in the inhibition of the parameters investigated.
Histaminergic drugs of the 1st generation inhibited oxidative burst of human phagocytes in whole blood and in isolated neutrophils. The rank order of potency to inhibit CL, MPO release and SO generation in PMA stimulated phagocytes was: Dit>Lor>His.
研究H1抗组胺药二硫氮卓（Dit）和氯雷他定（Lor）的作用，并将其与组胺（His）对佛波酯（PMA）刺激的全血、分离的中性粒细胞化学发光（CL）、髓过氧化物酶（MPO）释放以及超氧化物（SO）生成的作用进行比较。
采用鲁米诺和异鲁米诺增强的CL来测量氧化爆发，用分光光度法测定MPO（邻联茴香胺）和SO生成（超氧化物歧化酶抑制细胞色素c）。
Dit和Lor剂量依赖性地抑制全血的CL，并显著降低中性粒细胞胞外和胞内部位的氧化爆发。两种药物在比抑制SO低10倍的浓度下测试时，MPO的释放均减少。组胺（His）在抑制所研究参数方面的效果要差得多。
第一代组胺能药物抑制全血和分离的中性粒细胞中人吞噬细胞的氧化爆发。在PMA刺激的吞噬细胞中抑制CL、MPO释放和SO生成的效力顺序为：Dit>Lor>His。
