编码人假定泛素缀合酶E2Z（UBE2Z）的基因的克隆与特性分析

Cloning and characterization of a gene encoding the human putative ubiquitin conjugating enzyme E2Z (UBE2Z).

作者信息

Gu Xing, Zhao Feng, Zheng Mei, Fei Xiangwei, Chen Xudong, Huang Shengdong, Xie Yi, Mao Yumin

机构信息

State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, PR China.

出版信息

Mol Biol Rep. 2007 Sep;34(3):183-8. doi: 10.1007/s11033-006-9033-7. Epub 2006 Dec 12.

DOI:10.1007/s11033-006-9033-7

PMID:17160626

Abstract

Ubiquitination, the covalent attachment of the protein ubiquitin (Ub) to other cellular proteins, has been implicated in a number of important physiological processes. ubiquitin conjugating enzyme (E2) plays an important role in the ubiquitin system. Here we report the research about a human putative ubiquitin conjugating enzyme gene, UBE2Z. The length of UBE2Z cDNA is 3054 base pairs and contains an open reading frame encoding 246 amino acids. The UBE2Z gene was mapped to human chromosome 17q21.32 and consisted of 6 exons. RT-PCR showed that UBE2Z was widely expressed in human tissues, especially high in placenta, pancreas, spleen and testis. The UBE2Z-GFP fusion protein was located in both nucleus and cytoplasm of AD293 cells.

摘要

泛素化作用，即蛋白质泛素（Ub）与其他细胞蛋白的共价连接，已被证明参与了许多重要的生理过程。泛素结合酶（E2）在泛素系统中发挥着重要作用。在此，我们报告了关于一个人类假定泛素结合酶基因UBE2Z的研究。UBE2Z cDNA的长度为3054个碱基对，包含一个编码246个氨基酸的开放阅读框。UBE2Z基因被定位到人类染色体17q21.32，由6个外显子组成。逆转录聚合酶链反应（RT-PCR）表明，UBE2Z在人体组织中广泛表达，在胎盘、胰腺、脾脏和睾丸中表达尤其高。UBE2Z-绿色荧光蛋白（GFP）融合蛋白位于AD293细胞的细胞核和细胞质中。