Li Guangtao, Lü Hongyan, Zhou Yan, Jin Jian, Jiang Keyi, Peng Xiaozhong, Yuan Jiangang, Qiang Boqin
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS & PUMC, Chinese National Human Genome Center, Beijing 100005.
Chin Med Sci J. 2002 Mar;17(1):7-12.
To clone and identify the gene encoding human ubiquitin binding enzyme 2 and study its expression pattern.
According to the sequence of human EST, which is highly homologous to the mouse ubiquitin binding/conjugating enzyme (E2), primers were synthesized to screen the human fetal brain cDNA library. The gene was analyzed by bioinformatics technique and its expression pattern was studied by using multiple-tissue Northern blot.
Two cDNA clones encoding human ubiquitin conjugating enzyme have been isolated and identified. Both containing the ubiquitin conjugating domain, the 2 cDNA clones are 88% identical in amino acid sequences and splicing isoforms to each other only with an exon excised to form the short sequence. They belong to a highly conserved and widely expressed E2 enzyme family. Northern blot shows that they are expressed exclusively in adult human heart, placenta, and pancreas but no transcripts can be detected in brain, lung, liver, skeletal muscle or kidney.
The gene encoding human ubiquitin binding enzyme is expressed under temporal control. As a key enzyme in the degradation of proteins, ubiquitin conjugating enzymes play a central role in the expression regulation on the level of post-translation.
克隆并鉴定编码人泛素结合酶2的基因，研究其表达模式。
根据与小鼠泛素结合/缀合酶（E2）高度同源的人EST序列合成引物，筛选人胎脑cDNA文库。采用生物信息学技术对该基因进行分析，并利用多组织Northern印迹法研究其表达模式。
已分离并鉴定出两个编码人泛素缀合酶的cDNA克隆。这两个cDNA克隆均含有泛素缀合结构域，氨基酸序列同源性为88%，且彼此为剪接异构体，仅切除一个外显子以形成短序列。它们属于一个高度保守且广泛表达的E2酶家族。Northern印迹显示它们仅在成人心脏、胎盘和胰腺中表达，而在脑、肺、肝、骨骼肌或肾中未检测到转录本。
编码人泛素结合酶的基因在时间控制下表达。作为蛋白质降解中的关键酶，泛素缀合酶在翻译后水平的表达调控中起核心作用。
