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体外受精后微环境维持对胚胎培养的影响：顶部加载式小型培养箱与传统前加载式培养箱的比较

Effect of micro-environment maintenance on embryo culture after in-vitro fertilization: comparison of top-load mini incubator and conventional front-load incubator.

作者信息

Fujiwara Mutsuko, Takahashi Kentaro, Izuno Mika, Duan Ya Ru, Kazono Maya, Kimura Fuminori, Noda Yoichi

机构信息

Department of Obstetrics and Gynecology, Shiga University of Medical Science, Seta-Tsukinowa-cho, Ohtsu, Shiga, 520-2192, Japan.

出版信息

J Assist Reprod Genet. 2007 Jan;24(1):5-9. doi: 10.1007/s10815-006-9088-3. Epub 2006 Dec 13.

DOI:10.1007/s10815-006-9088-3
PMID:17160731
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3455086/
Abstract

PURPOSE

To investigate the effect of microenvironment maintenance on embryo culture and clinical results using two types of incubators.

METHODS

Temperature and oxygen concentration in a mini-incubator and a conventional incubator were compared following a 5-s door opening/closing procedure. Embryos of 30 in-vitro fertilization embryo transfer (IVF-ET) cases were randomly allocated to either one of the incubator, cultured, and the early-stage good embryo formation rate and the good blastocyst formation rate were compared, as indicators for micro-environment maintenance ability.

RESULTS

Temperature recovery after a 5-s door opening/closing procedure was approximately 5 min for the mini-incubator and 30 min for the conventional incubator. The oxygen concentration return was significantly improved in the mini-incubator (3.0 +/- 0 min) compared with the conventional incubator (7.8 +/- 0.9 min). Both the early-stage good embryo formation rate and the good blastocyst formation rate were significantly higher in the mini-incubator (39.5% and 15.1%) than the conventional incubator (28.4% and 7.8%).

CONCLUSION

The microenvironment maintenance ability of incubators appears to significantly influence the formation of good embryos.

摘要

目的

使用两种类型的培养箱研究微环境维持对胚胎培养及临床结果的影响。

方法

在5秒的开门/关门程序后，比较小型培养箱和传统培养箱中的温度和氧气浓度。将30例体外受精胚胎移植（IVF-ET）病例的胚胎随机分配至其中一种培养箱进行培养，比较早期优质胚胎形成率和优质囊胚形成率，作为微环境维持能力的指标。

结果

5秒开门/关门程序后的温度恢复时间，小型培养箱约为5分钟，传统培养箱约为30分钟。与传统培养箱（7.8±0.9分钟）相比，小型培养箱中的氧气浓度恢复显著改善（3.0±0分钟）。小型培养箱中的早期优质胚胎形成率（39.5%）和优质囊胚形成率（15.1%）均显著高于传统培养箱（28.4%和7.8%）。

结论

培养箱的微环境维持能力似乎对优质胚胎的形成有显著影响。

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