Yeung Queenie Sum Yee, Briton-Jones Christine May, Tjer Grace Ching Ching, Chiu Tony Tak Yu, Haines Christopher
Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong.
J Assist Reprod Genet. 2004 Oct;21(10):355-60. doi: 10.1023/b:jarg.0000046203.44045.0e.
To investigate whether commonly used test tube warming devices maintain a constant temperature in follicular fluid aspirates.
By using a digital thermocouple, temperature was measured and comparisons were made between an analog dry block heater, a digital dry block heater, and a thermostatic test tube heater.
For small fluid volumes, temperature in the block heaters increased above 37 degrees C after being in the block for over 2 min. The thermostatic heater maintained a constant temperature, but this was below the factory setting of 36.9 degrees C. Temperature maintenance was influenced by fluid volume in each tube.
One of the key factors in the handling of gametes and embryos is the maintenance of constant temperature. Test tube warming devices require verification of their ability to maintain fluid at the desired temperature. Temperature may vary with fluid volume and the type of test tube warming device used.
研究常用的试管加热装置能否使卵泡液抽吸物保持恒定温度。
使用数字热电偶测量温度,并对模拟干式恒温器、数字干式恒温器和恒温试管加热器进行比较。
对于少量液体,在恒温器中放置超过2分钟后,恒温器中的温度升高至37摄氏度以上。恒温加热器能保持恒定温度,但低于工厂设定的36.9摄氏度。每个试管中的温度维持受液体体积影响。
配子和胚胎处理中的关键因素之一是保持恒定温度。试管加热装置需要验证其将液体维持在所需温度的能力。温度可能随液体体积和所用试管加热装置的类型而变化。