纤维蛋白原Bβ -1420G/A、-993C/T和-854G/A基因多态性与冠心病的相关性
[Association of fibrinogen B beta -1420G/A, -993C/T and -854G/A gene polymorphism with coronary heart disease].
作者信息
Xing Hong-yun, Cai Wang-wei, Li Xiao-ming, Fu Qiao, Liu Guo-xun
机构信息
Department of Hematology, Affiliated Hospital, Luzhou Medical College, Luzhou, Sichuan, 646000, P. R. China.
出版信息
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2006 Dec;23(6):622-6.
OBJECTIVE
To analyze the frequency of FGB gene -1420G/A, -993C/T and -854G/A polymorphisms, and their association with plasma fibrinogen levels in patients with coronary heart disease and in health adults.
METHODS
The FGB gene -1420G/A, -993C/T and -854G/A polymorphisms were analyzed with restriction fragment length polymorphisms, polymerase chain reaction with allele-specific primer and nucleotide sequencing methods. Plasma fibrinogen levels were determined by turbidimetry.
RESULTS
The frequencies of -1420A were 0.33 in patients with coronary heart disease and 0.26 in health adults. The frequencies of -1420A in coronary heart disease were apparently higher than that in health adults. There were no difference in frequencies of other two alleles. The logistic study suggested -1420G/A polymorphism was associated with coronary heart disease. There are significantly difference in plasma fibrinogen levels in two groups. Plasma fibrinogen levels were significantly increased in patients with coronary heart disease.
CONCLUSION
This study suggests -1420G/A polymorphism may be associated with occurrence of coronary heart disease.
目的
分析冠心病患者及健康成人中FGB基因-1420G/A、-993C/T和-854G/A多态性的频率及其与血浆纤维蛋白原水平的关系。
方法
采用限制性片段长度多态性、等位基因特异性引物聚合酶链反应和核苷酸测序方法分析FGB基因-1420G/A、-993C/T和-854G/A多态性。采用比浊法测定血浆纤维蛋白原水平。
结果
冠心病患者中-1420A的频率为0.33，健康成人中为0.26。冠心病患者中-1420A的频率明显高于健康成人。其他两个等位基因的频率无差异。逻辑回归研究表明-1420G/A多态性与冠心病有关。两组血浆纤维蛋白原水平有显著差异。冠心病患者血浆纤维蛋白原水平显著升高。
结论
本研究提示-1420G/A多态性可能与冠心病的发生有关。
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