[中国成都汉族人群Y染色体两个短串联重复序列位点的多态性分析]
[Polymorphic analysis of two Y chromosome short tandem repeat loci in Chinese Chengdu Han population].
作者信息
Lin Jing-dong, Huang Ai-ming, Huang Jian-wen
机构信息
Department of Pathology, College of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, 350004, P. R. China.
出版信息
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2006 Dec;23(6):683-4.
OBJECTIVE
To reveal the distribution of genetic polymorphism of two novel Y chromosome short tandem repeat (Y-STR) loci (DYS508, DYS516) in Chinese Chengdu Han population.
METHODS
Two Y-STR loci were amplified with PCR. The products of PCR were analyzed with polyacrylamide gel electrophoresis. Each allele of these 2 Y-STR loci was sequenced and the allele ladders were constructed. Alleles of these Y-STR loci were nominated according to recommendations of the International Society of Forensic Genetics (ISFG).
RESULTS
Six and 6 alleles were observed in loci DYS508 and DYS516 respectively. The gene diversity of DYS508 and DYS516 was 0.7242 and 0.7931 respectively. The diversity of haplotype for these Y-STR loci was 0.9397. The value of discrimination power and the exclusion chance of paternity for these Y-STR loci were same as the value of haplotype diversity.
CONCLUSION
Both Y-STR loci are high genetic polymorphic. They are beneficial to the test of male-female mixtures and paternity identification in forensic science.
目的
揭示两个新型Y染色体短串联重复序列（Y-STR）基因座（DYS508、DYS516）在中国成都汉族人群中的遗传多态性分布情况。
方法
采用聚合酶链反应（PCR）扩增两个Y-STR基因座。PCR产物用聚丙烯酰胺凝胶电泳进行分析。对这2个Y-STR基因座的每个等位基因进行测序并构建等位基因阶梯。这些Y-STR基因座的等位基因根据国际法医遗传学协会（ISFG）的建议进行命名。
结果
在DYS508和DYS516基因座分别观察到6个和6个等位基因。DYS508和DYS516的基因多样性分别为0.7242和0.7931。这些Y-STR基因座的单倍型多样性为0.9397。这些Y-STR基因座的鉴别力值和父权排除率与单倍型多样性值相同。
结论
这两个Y-STR基因座均具有高度的遗传多态性。它们有利于法医学中男女混合样本检测和亲子鉴定。
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