[口蹄疫病毒A22亚株抗原决定簇编码人工基因的合成、克隆与表达]

[Synthesis, cloning, and expression of artificial genes, coding antigenic determinants of the foot-and-mouth virus substrain A22].

作者信息

Korobko V G, Boldyreva E F, Nekrasova O V, Mikul'skis A, Filippov S A, Dobrynin V N

出版信息

Bioorg Khim. 1991 Apr;17(4):461-9.

Abstract

Chemical-enzymatic synthesis and cloning in Escherichia coli of double-stranded DNAs, coding for simple and complex antigenic determinants of foot-and-mouth disease virus (FMDV) strain A22, have been carried out. The simple antigenic determinants are a part of the viral coat protein VP1 (amino acid sequence 131-152 or 131-160) whereas the complex antigenic determinants comprise additionally the amino acid sequence 200-213 of VP1 linked to N-terminus of simple antigenic determinants through a tetrapeptide spacer Pro-Pro-Ser-Pro. Recombinant DNAs containing genes for antigenic determinants of FMDV fused with C-terminus of gene for human tumor necrosis factor (hrTNF) have been constructed. Expression of the hybrid genes and properties of the proteins coded were studied. All recombinant proteins were shown to interact specifically with polyclonal antibodies both against hrTNF and FMDV strain A22. The recombinant proteins produced by bacteria are perspective for study as a vaccine against FMDV.

摘要

已开展了口蹄疫病毒（FMDV）A22株简单和复杂抗原决定簇编码双链DNA的化学酶促合成及在大肠杆菌中的克隆。简单抗原决定簇是病毒衣壳蛋白VP1的一部分（氨基酸序列131 - 152或131 - 160），而复杂抗原决定簇还包括VP1的氨基酸序列200 - 213，其通过四肽间隔区Pro - Pro - Ser - Pro与简单抗原决定簇的N端相连。构建了含有与人类肿瘤坏死因子（hrTNF）基因C端融合的FMDV抗原决定簇基因的重组DNA。研究了杂交基因的表达及所编码蛋白质的特性。所有重组蛋白均显示与抗hrTNF和FMDV A22株的多克隆抗体发生特异性相互作用。细菌产生的重组蛋白有望作为抗FMDV疫苗进行研究。