Crevillén Agustín González, Blasco Antonio Javier, González María Cristina, Escarpa Alberto
University of Alcalá, Madrid, Spain.
Electrophoresis. 2006 Dec;27(24):5110-8. doi: 10.1002/elps.200600213.
A novel analytical route to determine water-soluble vitamins (B group and C) using single channel microchip-electrochemistry platforms is presented. The electrochemical detection protocol was carefully optimized, and it was shown that it was crucial to use 1 M nitric acid in the detector compartment to detect folic acid. A phosphate buffer (pH 6, 10 mM) and a separation voltage of 2 kV gave the complete separation of vitamins in less than 130 s, with good reproducibility (RSDs less than 10%) and accuracy (error less than 9%). In addition, a methodological innovation integrating calibration and analysis of water-soluble vitamins on the chip is also proposed. The strategy consisted in sequentially using both reservoirs (named calibration and analysis reservoirs) as well as a calibration factor (defined as signal/concentration of analyte). The analytical route required 350 s in the overall protocol (employing 130 s in calibration plus 130 s in analysis), an improvement over the times used in both conventional and microchip protocols.
本文介绍了一种使用单通道微芯片电化学平台测定水溶性维生素（B族和C族）的新型分析方法。对电化学检测方案进行了仔细优化，结果表明在检测池中使用1 M硝酸对于检测叶酸至关重要。磷酸盐缓冲液（pH 6，10 mM）和2 kV的分离电压可在不到130 s的时间内实现维生素的完全分离，具有良好的重现性（相对标准偏差小于10%）和准确性（误差小于9%）。此外，还提出了一种在芯片上集成水溶性维生素校准和分析的方法创新。该策略包括依次使用两个储液器（分别称为校准储液器和分析储液器）以及一个校准因子（定义为信号/分析物浓度）。整个分析流程在总体方案中需要350 s（校准用时130 s加上分析用时130 s），相较于传统方法和微芯片方法所用时间有所改进。
