Li Xiang-Yun, Zhang Qian-Li, Lian Hong-Zhen, Xu Jing-Juan, Chen Hong-Yuan
Key Lab of Analytical Chemistry for Life Science, Ministry of Education, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing, China.
J Sep Sci. 2007 Sep;30(14):2320-5. doi: 10.1002/jssc.200700155.
A method for rapid separation and sensitive determination of three water-soluble vitamins, pyridoxine, ascorbic acid (VC), and p-aminobenzoic acid (PABA) has been developed by PDMS microchannel electrophoresis integrated with amperometric detection. After treatment of the microchip with oxygen plasma, the peak shapes of the three analytes were essentially improved. Pyridoxine, VC, and PABA were well separated within only 80 s in a running buffer of 20 mM borate solution (pH 8.5). Good linearity was obtained within the concentration range of 2-200 microM for the three water-soluble vitamins. The detection limits were 1.0 microM for pyridoxine and VC, and 1.5 microM for PABA. The proposed method has been successfully applied to real human urine sample, without solid phase extraction, with recoveries of 80-122% for the three water-soluble vitamins.
