Ivanov B B, Iurovskiĭ V V, Tertyshnikova S M, Andronova T M, Ivanov V T
Bioorg Khim. 1991 Apr;17(4):486-93.
To study coat proteins of Plasmodium falciparum, seven putative antigenic-determinants of PMMSA, SHARP, GBP and four known determinants of CSP, CRA and RESA were synthesized. Computerized methods for predicting protein antigenic determinants were employed to select the peptides. For immunochemical studies the peptides were conjugated to proteins and synthetic carriers by means of nonspecific and regiospecific heterobifunctional reagents.
为研究恶性疟原虫的表面蛋白，合成了疟原虫膜表面蛋白1（PMMSA）、富含半胱氨酸的酸性蛋白（SHARP）、富含甘氨酸的蛋白（GBP）的七个假定抗原决定簇以及环子孢子蛋白（CSP）、富含半胱氨酸的富含丙氨酸的蛋白（CRA）和富含半胱氨酸的红细胞膜内蛋白（RESA）的四个已知抗原决定簇。采用计算机化方法预测蛋白质抗原决定簇以选择肽段。为进行免疫化学研究，通过非特异性和区域特异性异双功能试剂将肽段与蛋白质及合成载体偶联。
