Wiesinger H, Schuricht B, Hamprecht B
Physiologisch-chemisches Institut der Universität, Tübingen, F.R.G.
Brain Res. 1991 May 31;550(1):69-76. doi: 10.1016/0006-8993(91)90406-l.
Primary cultures derived from the brains of newborn mice are quantitatively dominated by astroglial cells, but contain also oligodendroglial, phagocytic and ependymal cells. When confluent cultures are fed with glucose-free growth medium containing 25 mM sorbitol for 14 days, oligodendroglial, phagocytic and ependymal cells are eliminated from the culture, as judged by morphological and immunocytochemical criteria. The remaining cells stain positively for vimentin and glial fibrillary acidic protein and, therefore, can be considered as astroglial cells. Inoculation of freshly dissociated mouse brain cells in the absence of glucose in a sorbitol-containing medium is not possible; however, feeding of the cultures from day 2 on with sorbitol instead of glucose results in a pure astroglial culture at confluency. Therefore glucose-free growth medium supplemented with sorbitol can be considered a selective medium for astroglial cells in primary mouse glial cultures.
源自新生小鼠大脑的原代培养物在数量上以星形胶质细胞为主，但也包含少突胶质细胞、吞噬细胞和室管膜细胞。当汇合培养物用含有25 mM山梨醇的无糖生长培养基培养14天时，根据形态学和免疫细胞化学标准判断，少突胶质细胞、吞噬细胞和室管膜细胞从培养物中被清除。剩余的细胞波形蛋白和胶质纤维酸性蛋白染色呈阳性，因此可被视为星形胶质细胞。在不含葡萄糖的含山梨醇培养基中接种新鲜解离的小鼠脑细胞是不可能的；然而，从第2天开始用山梨醇代替葡萄糖培养培养物，汇合时可得到纯星形胶质细胞培养物。因此，补充了山梨醇的无糖生长培养基可被视为原代小鼠胶质细胞培养中星形胶质细胞的选择性培养基。
