Hansson E
Neurochem Res. 1984 Feb;9(2):153-72. doi: 10.1007/BF00964164.
In this overview attention is given to available markers and methods for characterizing cell elements in a culture system. Primary cultures from newborn rat cerebral hemispheres were grown for 14 days. The population of cells was dominated by astrocytic glial cells (60-70%), but cells with properties of macrophages, endothelial-like cells, mesenchymal-like cells, ependymal-like cells, and oligoblasts were also found. Neither mature neurons nor oligodendroglial cells were observed. The enrichment in astroglial-like cells makes the cultures a satisfactory astroglial-cell model, at least for some purposes.
在本综述中，我们关注了用于表征培养系统中细胞成分的现有标志物和方法。新生大鼠脑半球的原代培养物培养了14天。细胞群体以星形胶质细胞为主（60 - 70%），但也发现了具有巨噬细胞、内皮样细胞、间充质样细胞、室管膜样细胞和少突胶质前体细胞特性的细胞。未观察到成熟神经元和少突胶质细胞。星形胶质样细胞的富集使得这些培养物成为一个令人满意的星形胶质细胞模型，至少对于某些目的而言是如此。
