Wang Chun-Chin, Lee Chi-Mei
Department of Environmental Engineering, Hungkuang University, Shalu, Taichung 433, Taiwan.
J Hazard Mater. 2007 Jun 25;145(1-2):136-41. doi: 10.1016/j.jhazmat.2006.10.092. Epub 2006 Nov 10.
epsilon-Caprolactam has high COD and toxicity, so its discharge to natural water and soil systems may lead to an adverse environmental effect on water quality, endangering public health and welfare. This investigation attempts to isolate epsilon-caprolactam denitrifying bacteria from a wastewater treatment system manufactured with acrylonitrile-butadiene-styrene (ABS) resin. The goal is to elucidate the effectiveness of isolated pure strain and ABS mixed strains in treating epsilon-caprolactam from synthetic wastewater. The results reveal that Paracoccus versutus MDC-3 was isolated from the wastewater treatment system manufactured with ABS resin. The ABS mixed strains and P. versutus MDC-3 can consume up to 1539mg/l epsilon-caprolactam to denitrify from synthetic wastewater. Complete epsilon-caprolactam removal depended on the supply of sufficient electron acceptors (nitrate). Strain P. versutus MDC-3, Hyphomicrobium sp. HM, Methylosinus pucelana and Magnetospirillum sp. CC-26 are related closely, according to the phylogenetic analyses of 16S rDNA sequences.
ε-己内酰胺具有高化学需氧量和毒性，因此将其排放到天然水和土壤系统中可能会对水质产生不利的环境影响，危及公众健康和福利。本研究试图从用丙烯腈-丁二烯-苯乙烯（ABS）树脂制造的废水处理系统中分离出ε-己内酰胺反硝化细菌。目的是阐明分离出的纯菌株和ABS混合菌株处理合成废水中ε-己内酰胺的效果。结果表明，从用ABS树脂制造的废水处理系统中分离出了维氏副球菌MDC-3。ABS混合菌株和维氏副球菌MDC-3能够消耗高达1539mg/l的ε-己内酰胺，从而对合成废水进行反硝化。完全去除ε-己内酰胺取决于充足电子受体（硝酸盐）的供应。根据16S rDNA序列的系统发育分析，维氏副球菌MDC-3菌株、生丝微菌属HM菌株、普氏甲基弯曲菌和趋磁螺菌属CC-26菌株亲缘关系密切。
