Thomas P A, Kuriakose T, Kirupashanker M P, Maharajan V S
Department of Microbiology, Joseph Eye Hospital, Tiruchirapalli, India.
Diagn Microbiol Infect Dis. 1991 May-Jun;14(3):219-24. doi: 10.1016/0732-8893(91)90035-e.
Lactophenol cotton blue (LPCB) mounts of corneal ulcer scrapings were assessed as a diagnostic tool in cases of mycotic keratitis over a period of 15 months. We investigated 568 cases of ulcerative keratitis by microbiological techniques consisting of direct microscopic examination of LPCB mounts and Gram-stained smears as well as culture of material scraped from the ulcer. Fungi were isolated in large numbers on multiple media from the corneal scrapings of 179 patients (culture-proven mycotic keratitis). Direct microscopic examination of LPCB mounts of corneal scrapings yielded positive results in 140 (78%) of 179 culture-positive patients and negative results in 371 (95%) of 389 culture-negative patients. There was a significant difference between the percentage of positive results obtained by LPCB mounts and by Gram-stained smears in the culture-positive cases. The LPCB mount was positive in greater than 80% of cases of keratitis due to Fusarium spp. and Aspergillus spp. The LPCB mount is strongly recommended as a simple, rapid, inexpensive, and sensitive diagnostic technique in cases of mycotic keratitis.
在15个月的时间里，对角膜溃疡刮片的乳酚棉蓝（LPCB）涂片进行评估，作为真菌性角膜炎病例的一种诊断工具。我们采用微生物学技术对568例溃疡性角膜炎病例进行了调查，这些技术包括对LPCB涂片和革兰氏染色涂片进行直接显微镜检查，以及对从溃疡处刮取的材料进行培养。从179例患者的角膜刮片中在多种培养基上大量分离出真菌（培养证实为真菌性角膜炎）。对角膜刮片的LPCB涂片进行直接显微镜检查，在179例培养阳性患者中有140例（78%）结果为阳性，在389例培养阴性患者中有371例（95%）结果为阴性。在培养阳性病例中，LPCB涂片和革兰氏染色涂片获得的阳性结果百分比之间存在显著差异。对于由镰刀菌属和曲霉菌属引起的角膜炎病例，LPCB涂片在超过80%的病例中呈阳性。强烈推荐LPCB涂片作为真菌性角膜炎病例的一种简单、快速、廉价且敏感的诊断技术。
