Katzenellenbogen E, Romanowska E, Dabrowski U, Dabrowski J
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Wroclaw, Poland.
Eur J Biochem. 1991 Sep 1;200(2):401-7. doi: 10.1111/j.1432-1033.1991.tb16198.x.
The Hafnia alvei strain 1211 O-specific polysaccharide is composed of 3-amino-N-(D-3'-hydroxybutyryl)-3,6-dideoxy-D-galactose, N-acetyl-D-galactosamine, N-acetyl-D-glucosamine and D-glucose (1:1:2:2). On the basis of sugar and methylation analyses, Smith degradation, and one- and two-dimensional 1H- and 13C-NMR spectroscopy, the polysaccharide was shown to be an O-acetylated polymer of the repeating hexasaccharide unit, ----2D(4-OAc)Fucp3NAcyl beta 1----6DGlcpNAc alpha 1---- (DGlcp beta 1----3)4DGalpNAc alpha 1----3DGlcpNAc beta 1----2DGlcp beta 1----, where DFucp3NAcyl = 3-amino-N-(D-3'-hydroxybutyryl)-3,6-dideoxy-D- galactopyranose. The O-specific polysaccharide showed some microheterogeneity due to incomplete substitution by terminal glucose.
蜂房哈夫尼亚菌1211菌株O-特异性多糖由3-氨基-N-（D-3'-羟基丁酰基）-3,6-二脱氧-D-半乳糖、N-乙酰-D-半乳糖胺、N-乙酰-D-葡萄糖胺和D-葡萄糖（1:1:2:2）组成。基于糖和甲基化分析、史密斯降解以及一维和二维1H-和13C-核磁共振光谱，该多糖被证明是重复六糖单元的O-乙酰化聚合物，----2D(4-OAc)Fucp3NAcyl β1----6DGlcpNAc α1----(DGlcp β1----3)4DGalpNAc α1----3DGlcpNAc β1----2DGlcp β1----，其中DFucp3NAcyl = 3-氨基-N-（D-3'-羟基丁酰基）-3,6-二脱氧-D-吡喃半乳糖。由于末端葡萄糖的不完全取代，O-特异性多糖表现出一些微观异质性。
