Takahashi K, Hata J, Mukai K, Sawasaki Y
Department of Biochemistry, National Defense Medical College, Saitama, Japan.
In Vitro Cell Dev Biol. 1991 Jul;27A(7):542-8. doi: 10.1007/BF02631284.
The mesothelial cells obtained from human omental adipose tissue showed a typical cobblestone monolayer and reacted strongly with keratin, but did not have Von Willebrand factor. Ultrastructurally these cells revealed the existence of desmosome-like cell junctions as well as intracellular canaliculi, tubular structures surrounded by microvilli, and tonofilament-like filaments. The mesothelial cells grew much faster in the medium containing epidermal growth factor, actively took up acetylated-low density lipoprotein into their cytoplasm, and released angiotensin-converting enzyme. They also released urokinase-type plasminogen activator, but only half as much as do human umbilical vein endothelial cells; release of tissue-type plasminogen activator was not observed. Inasmuch as the mesothelial cells also released plasminogen activator inhibitor-1, as do human umbilical vein endothelial cells, we could not detect u-PA activity in culture medium. u-Pa may play a role in the protection against adhesion among visceral organs. These observations indicate that cultured human mesothelial cells have characteristics closely related to those found in human endothelial cells.
从人网膜脂肪组织获取的间皮细胞呈现出典型的鹅卵石样单层结构，与角蛋白反应强烈，但不含有血管性血友病因子。超微结构显示，这些细胞存在桥粒样细胞连接以及细胞内小管、被微绒毛包围的管状结构和张力丝样细丝。间皮细胞在含有表皮生长因子的培养基中生长得更快，能主动将乙酰化低密度脂蛋白摄取到细胞质中，并释放血管紧张素转换酶。它们还释放尿激酶型纤溶酶原激活物，但释放量仅为人脐静脉内皮细胞的一半；未观察到组织型纤溶酶原激活物的释放。由于间皮细胞也像人脐静脉内皮细胞一样释放纤溶酶原激活物抑制剂-1，所以我们在培养基中检测不到尿激酶型纤溶酶原激活物的活性。尿激酶型纤溶酶原激活物可能在内脏器官间粘连的预防中发挥作用。这些观察结果表明，培养的人源间皮细胞具有与人类内皮细胞密切相关的特征。
